"History will remember this day as a day in which new paths of hope were opened toward the European deal," Portuguese Prime Minister Jose Socrates told the ceremony.
He insisted the treaty -- which replaces a planned EU constitution scuppered by French and Dutch referendums in 2005 -- is not a threat to the national sovereignty of member states.
The Treaty of Lisbon was only agreed after long and often acrimonious negotiations between supporters and opponents of closer integration however.
And the changes are likely to face new 'eurosceptic' opposition when they are discussed in national parliaments that have to ratify the treaty. Ireland has to hold a referendum.
EU leaders, who move on to a summit in Brussels on Friday, deem it vital to streamline the functioning of the bloc, which has grown from 15 to 27 nations since 2004 while pushing deep into the former Soviet bloc.
Like the rejected constitution, it proposes a European foreign policy supremo and a permanent president to replace the cumbersome six-month rotating presidency system.
It cuts the size of the European Parliament and the number of EU decisions which require unanimous support, hence reducing national vetoes.
However it drops all references to an EU flag or anthem, to assuage eurosceptic fears of another step towards a federal Europe.
It also includes a European charter of fundamental human and legal rights, which Britain and Poland have refused to make binding.
"With the Treaty of Lisbon, Europe finally overcomes the political and institutional impasse that limited its capacity to act during the last few years," Socrates said.
"The European project does not eliminate nor minimise national identities," he insisted.
EU Commission Chief Jose Manuel Barroso said "the enlarged European Union gives us a new economic, political and strategic dimension."
"This dimension makes each member state stronger. And it makes Europe, united in its diversity, better equipped to promote its interests and values in the world," he added.
British Prime Minister Gordon Brown was a notable exception at the signing ceremony at Lisbon's Jeronimos Monastery. Brown appeared before a parliamentary committee in London before joining his fellow EU leaders to sign the 250-plus page text.
His failure to attend the public celebration of European unity led to accusations in Britain of a luke-warm attitude to Europe.
But he rejected the charges. "I think you'll find on the debate about global Europe, we are leading the way," he said in The Times newspaper.
At the signing of the rights charter in the European Parliament on Wednesday, Socrates had to contend with far-left and right lawmakers chanting "referendum," a sign that the treaty signing is not the end of the debate.
Member states now have one year to ratify the text, if it is to come into force as planned in January 2009.
Many governments, including France and the Netherlands, have said they will not hold national referendums this time.
However opponents who want a national vote have seized on the words of Valery Giscard d'Estaing, France's former president and architecht of the abandoned European constitution.
He has said that legal experts had "taken the original draft constitution, blown it apart into separate elements, and have then attached them, one by one, to existing treaties."
Only Ireland is constitutionally bound to put the issue to a vote, which is expected in May or June. Polls suggest many Irish voters are undecided.
In order to avoid a referendum, the British government was granted key opt-outs on foreign policy, labour rights, the common law and tax and social security systems.
At Friday's summit in Brussels, the leaders are expected to move on to discuss Iran, Kosovo and globalisation.
Güncelleme Tarihi: 14 Aralık 2007, 10:07