A senior diplomat from Slovenia, which holds the rotating presidency of the European Union, was also summoned, the radio said. The Netherlands is a member of the 27-nation bloc.
Iran has denounced the film by Dutch lawmaker Geert Wilders as "heinous" and called on European governments to block any further showing.
"Following the propagation of the insulting and anti-Islamic film by the radical Dutch parliamentarian, the Dutch ambassador and the Slovenian charge d'affaires were summoned to the Foreign Ministry," state radio said.
Wilders, leader of the anti-immigration Freedom Party, launched his short video on the Internet on Thursday, prompting protests from Muslims and states.
The film "Fitna" -- an Arabic term sometimes translated as "strife" -- intersperses images of the Sept. 11, 2001, attacks on the United States and bombings with quotations from the Holy Koran.
Güncelleme Tarihi: 30 Mart 2008, 16:53